TOTAL VIEWS: 4667
This study examines the preparation, preliminary characterization and antioxi-dant abilities of Opuntia dillenii Haw polysaccharides-gelatin microspheres (ODP-GMS). ODP-GMS was prepared through W/O emulsion chemicalcros-sline method.The result shows that under the optimal conditions which a wa-ter/oil (W/O) ratio of 1 to 3.67, 1.06% emulsifier content and 11.47 mg/mL Opuntia dillenii Haw polysaccharides (ODP), the size of the spheroid ODP-GMS was relatively homogeneous and the diameter exhibited a normal distribution curve. The diameter of the microspheres increased by 22.81±0.45% after they were exposed to water for 2 hours. The accumulative release rate of ODP-GMS reached 90%in HCl over 12 hours, while the release rate was 85% in PBS over 24 hours. ODP-GMS had strong scavenging effects on three types of free-radicals (1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radicals, and superoxide radical). These results suggested that gelatin-based microspheres offer excellent potential as carriers for the controlled release of ODP in functional foods or medicine.
[1] Shirazinia R, Kehkhaie S, Rakhshandeh A. Opuntia dillenii: a forgotten plant with promising pharmacological properties. J Pharmacopuncture, 2019, 22(1) 16-27. doi: 10.3831/KPI.2019.22.002.
[2] Zhao L.Y.; Huang W.; Yuan Q.X.; Cheng J.; Huang Z.C.; Ouyang L.J.; Zeng F.H. Hypolipidemic effects and mechanisms of the main component of Opuntia dillenii Haw. polysaccharides in high-fat emulsion-induced hyperlipidemic rats. Food Chem. 2012, 134(2), 964-971.
[3] Zhao L.Y.; Lan Q.J.; Huang Z.C.; Ouyang L.J.; Zeng F.H. Antidiabetic effect of a newly identified component of Opuntia dillenii Polysaccharides. Phytomedicine. 2011, 18(8-9), 661-668.
[4] Carocho M.; and Ferreira IC. A review on antioxidants, prooxidants and related controversy: Natural and synthetic compounds, screening and analysis methodologies and future perspectives. Food Chem Toxicol. 2013, 51(1), 15-25.
[5] Vandelli M.A.; Rivasi F.; Guerra P.; Forni F.; Arletti R. Gelatin microspheres crosslinked with D,L-glyceraldehyde as a potential drug delivery system: preparation characterisation, in vitro and in vivo studies. Int J Pharmaceut. 2001, 215(1-2), 175-184.
[6] Sun R.; Shi J.J.; Guo Y.C.; Ch L.J. Studies on the paticle size control of gelatin microspheres. Front Chemistry. 442 China. 2009, 4(2), 222-228.
[7] Torrado J.J.; Illurn L.; Davis S.S. Particle size and size distribution of albumin microspheres produced by heat and chemical stabilization. Int J Pharmaceut. 1898, 51(89), 85-93.
[8] Rokhade A.P.; Agnihotri S.A.; Patil S.A.; Mallikarjuna N.N.; Kulkarni P.V.; Aminabhavi T.M. Semi-interpenetrating polymer network microspheres of gelatin and sodium carboxymethyl cellulose for controlled release of ketorolac tromethamine. Carbohyd Polym. 2006, 65(3), 243-252.
[9] Dunne M.; Corrigan O.I.; Ramtoola Z. Influence of particle size and dissolution conditions on the degradation properties of polylactide-co-glycolide particles. Biomaterial. 2000, 21(16), 1659-1668.
[10] Peng Z.Y.; Li Z.P.; Shen Y.Q. Influence of Chemical Cross-Linking on Properties of Gelatin/Chitosan Microspheres. Polym-Plast Technol. 2012, 51(4), 381-385.
[11] Genta I.; Costantini M.; Asti A.; Conti B.; Montanari L. Influence of glutaraldehyde on drug release and mucoadhesive properties of chitosan microspheres. Carbohyd Polym. 1998, 36(2), 81-88.
[12] Ubaidulla U.; Sultana Y.; Ahmed F.J.; Khar R.K.; Panda A.K. Chitosan Phthalate Microspheres for Oral Delivery of Insulin: Preparation, Characterization, and In Vitro Evaluation. Drug Deliv. 2007, 14(1), 19-23.
[13] Park J.M.; Lee S.Y.; Lee G.H.; Chung E.Y.; Chang K.M.; Kwak B.K.; Kuh H.J.; Lee J. Design and characterisation of doxorubicin-releasing chitosan microspheres for anti-cancer chemoembolisation. J Microencapsul. 2012, 29(7), 695-705.
[14] Esposito E.; Cortesi R.; Nastruzzi C. Gelatin microspheres: influence of preparation parameters and thermal treatment on chemico-physical and biopharmaceutical properties. Biomaterials. 1996, 17(20), 2009-2017.
[15] Ai S.; Fan X.D.; Fan L.; Sun Q.; LiuY.; Tao X.F.; Dai K. Extraction and chemical characterization of Angelica sinensis polysaccharides and its antioxidant activity. Carbohyd Polym. 2013, 94(2), 731-736.
[16] Rangika W.; Peter F.; Shantha L.K. Chitosan microspheres for encapsulation of a-lipoic acid. Int J Pharmaceut. 2008, 357, 213-218.
[17] Li H, Yuan Q, Zhou X, Zeng F, Lu X. Extraction of Opuntia dillenii Haw. polysaccharides and their antioxidant activities. Molecules. 2016, 21, 1612-1616. doi:10.3390/molecules21121612.
[18] Lupo B.; Maestro A.; Porras M.; Gutiérrez J.M.; González C. Preparation of alginate microspheres by 435 emulsification/internal gelation to encapsulate cocoa polyphenols. Food Hydrocolloid. 2014, 38(3), 56-65.
[19] Noda Y.; Asada C.; Sasaki C.; Saki H., Nakamura Y. Extraction method for increasing antioxidant activity of raw garlic using steam explosion. Biochem Eng J. 2013, 73(8), 1-4.
Preparation and Characterization of Opuntia dillenii Haw. Polysaccharide Gelatin Microspheres
How to cite this paper: Heng Li, Jie Cheng, Fuhua Zeng. (2022) Preparation and Characterization of Opuntia dillenii Haw. Polysaccharide Gelatin Microspheres. International Journal of Clinical and Experimental Medicine Research, 6(4), 455-464.
DOI: http://dx.doi.org/10.26855/ijcemr.2022.10.022