Article http://dx.doi.org/10.26855/ijcemr.2022.10.024

Atomic Force Microscopy Study of Human Dentin Collagen Fibrils


Jiajun Qian1, Miaomiao Chen1, Xuhai Weng1, Fengyong Wang2, Jianfeng Shi2, Haibin Ni2,*

1Department of dental, Nanxun District Lianshi People's Hospital of Huzhou City, Zhejiang Province, China, 313013.

2Department of dental, Tongde Hospital of Zhejiang Province, Zhejiang Province, China, 310012.

*Corresponding author: Haibin Ni

Published: November 24,2022


Objective: The aim of this study is to observe human dentin collagen fibrils with Atomic force microscopy (AFM) in air. Methods: Freshly extracted caries-free human third molars were collected and stored in normal saline at 4℃ until prepared. The midcoronal dentin specimens were sectioned and wet polished through a series of SiC papers, then the specimens were etched with 10%(w/w) citric acid for 15s and further treated with an aqueous solution of 10vol% NaOCl as deproteinizing agent for 120s to remove the non-collagenous proteins. Subsequently, the topography of dentin specimens was measured with AFM in air. Results: Images of dentin collagen fibrils were observed. Dentin collagen fibrils interweaved into a network in the intertubular dentin, and a cross-banding periodicity along collagen fibrils was about 67nm. Conclusions:  Clear images of dentin collagen fibrils were obtained with AFM and the detailed structures of dentin collagen fibrils were measured accurately. AFM is a useful tool for real-time observation of dentin collagen fibrils.


[1] Trache A, Meininger GA. Atomic force microscope (AFM). Curr Protoc Microbiol, 2008 Feb; chapter 2; Unit 2C.2.

[2] M. Balooch, S Habelitz, J.H.Kinney, et al. Mechanical properties of mineralized collagen fibrils as influenced by demineralization. Struct boil, 2008 Jun, 162(3):404-10.

[3] M. Di Renzo, T.H. Ellis, E Sacher, et al. A photoacoustic FTIRS study of the chemical modifications of human dentin surfaces: II. Deproteination. Biomaterials, 2001, 22:793-797.

[4] GW Marshall, Jr., N.Yücel, et al. Sodium hypochlorite alterations of dentin and dentin collagen. Surface Sci, 2001. 

[5] Bin Yang, Rainer Adelung, Klaus Ludwig, et al. Effect of structural change of collagen fibrils on the durability of dentin bonding. Biomaterials, 2005, 26:5021-5031.

[6] Stefan Habelitz, Mehdi Balooch, Sally J. Marshall, et al. In situ atomic force microscopy of partially demineralized human dentin collagen fibrils. Journal of Structural Biology, 2002, 138:227-236.

[7] F.El Feninata, T.H.Ellisa, E.Sacherb, et al. A tapping mode AFM study of collapse and denaturation in dentinal collagen. Dental Materials, 2001, 17:284-288.

[8] Wess TJ. Collagen fibril form and function. Adv Protein Chem, 2005, 70:341-74.

[9] Tim J. Wess, Joseph P. Orgel. Changes in collagen structure: drying, dehydrothermal treatment and relation to long term deterioration. Thermochimica Acta, 2000, 365: 119-128.

How to cite this paper

Atomic Force Microscopy Study of Human Dentin Collagen Fibrils

How to cite this paper: Jiajun Qian, Miaomiao Chen, Xuhai Weng, Fengyong Wang, Jianfeng Shi, Haibin Ni. (2022) Atomic Force Microscopy Study of Human Dentin Collagen Fibrils. International Journal of Clinical and Experimental Medicine Research6(4), 469-475.

DOI: http://dx.doi.org/10.26855/ijcemr.2022.10.024