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Article http://dx.doi.org/10.26855/ijfsa.2023.03.013

Effects of Variety and Fungicides on Germination Percentage and Seedling Vigor of Rice (Oryza sativa L.) Seeds

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C. C. Iwuagwu1,*, P. C. Ozofor1, U. O. Aguwa1, D. E. Iheaturu1, O. A. Apalowo1, M. E. Ejiofor1, D. C. Iwu2

1Department of Crop Science and Horticulture, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria.

2Department of Vocational Education, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria.

*Corresponding author: C. C. Iwuagwu

Published: March 22,2023

Abstract

A laboratory experiment was conducted to test the effects of variety and fungicides on percentage germination and seedling vigor of rice seeds. The experiment was conducted at Crop Science and Horticulture Laboratory, Nnamdi Azikiwe University, Awka, Anambra State. The experiment was a 2 x 4 factorial laid out in a Completely Randomized Design (CRD) and replicated three times. The result showed that there was significant effect of fungicide on percentage germination of rice seeds. There was no significant effect of variety on percentage germination of rice seeds where the highest percentage germination of 86.7% was obtained in Faro 44, which was statistically same with 69.0% obtained in Faro 52. The result also showed that the different fungicides had significant effect on germination of rice seeds where the crude extract of Aidan fruit pulp gave the highest (91.3%) germination. The same was also obtained in crude extract of Aidan fruit seed which is statistically different from 88.7% seed germination of rice obtained in Mancozeb and 40.0% obtained in Dressforce (D). It was also observed that the least percentage germination (40.0) was obtained in rice seed dressed with Dressforce (D). Both the plant extract and synthetic fungicide where effective in protecting the price seeds, though the plant extract had higher germination index than synthetic fungicide. Various fungicide had significant effect on vigor index of rice seeds where Mancozeb (M) had the highest seedling vigor index (34.0) followed by 19.2 obtained in rice seeds dressed with plant extract from seed of Aidan fruit while the least (9.2) obtained in rice seeds dressed with Dressforce (D). Generally, rice seeds dressed with Mancozeb gave highest seedling vigor index than the other fungicides. From this experiment it was observed that Dressforce had low germination because of the phytotoxic effect of the chemical on the rice seeds. There was no significant effect of the different fungicides used on percentage infection of rice seeds though rice seeds dressed with seed extract of Aidan fruit gave the highest percentage infection (6.67%) which was followed by pulp extract (4.0%) while the Dressforce and Mancozeb gave no infection. It is therefore recommended that for higher percent germination of rice seeds fungicides from crude extracts of Aiden fruit should be used but the synthetic fungicides-Dressforce and Mancozeb should be used for reduced fungi infection.

References

[1] Vaughan DA. (1994). The wild relatives of rice: a genetic resources handbook. International Rice Research Institute, Manila, p. 137.

[2] FAO, STAT. (2013). FAOSTAT Database. Food and Agriculture Organization of the United Nations, Rome, Italy.

http://faostat.fao.org.

[3] Pandey, S. and Velasco, L. (1999). Economics of Direct Seeding in Asia: Patterns of Adoption and Research Priorities. Rice Notes, 24, 6-11.

[4] IRRI. (2002) Standard Evaluation System for Rice (SES). International Rice Research Institute, Manila. 

http://www.knowledgebank.irri.org/images/docs/rice-standard- evaluation- system.pdf.

[5] Marcos-Filho, J. (2015). Seed vigor testing: an overview of the past, present and future perspective. Scientia Agricola., 72: 363-374.

[6] Utobo E B, Ogbodo E N, and Nwogbaga A C. (2011). Seed borne mycota associated with rice and their influence on growth of Abakaliki, Southeast agro-ecology, Nigeria. Libiyan Agric Res Cen J Inter, 2, 79-84.

[7] Ahmed M, Hossain M, Hassan K, and Dash C K. (2013). Efficacy of different plant extract on reducing seed borne infection and increasing germination of collected rice seed sample. Universal J of Plant Sci., 1(3), 66-73.

[8] Upadhyay, P. and Singh, S. P. (2019). Detection Methods for Seed Borne Pathogens. Int. J. Curr. Microbiol. App. Sci., (2019), 8(3): 318-323. https://doi.org/10.20546/ijcmas.2019.803.039.

[9] Fakir, G. A. (2000). An Annotated List of Seed-Borne Disease in Bangladesh. Seed Pathology Centre, Dept. of Plant Pathology, Bangladesh Agricultural University, Mymensingh.

[10] ISTA. (1996). International Rules for Seed Testing. Seed Sci. Technol., 24: 39-42.

[11] Basak A B and Mridha M A U. (1985). Mycoflora associated with seeds of different varieties Aman rice collected from Chit-tagong and Chittagong Hill-tract districts of Bangladesh. Seed res., 13(2), 78-84.

[12] Basak AB, Mrida MAU, and Fakir GA. (1991). Mycoflora of chilli seeds as isolated from different types of fruit rot occurring in chittangong district, Chittagong University Stud. Publication II, Science.

[13] Ou, S.H. (1985). Rice Diseases. 2nd Edition, Commonwealth Mycological Institute, Kew, 380 p.

[14] Grewal, A.S., Singla, A, Kamboj, P. Dua, J.S. (2017). Pesticide Residues in Food Grains, Vegetables and Fruits: A Hazard to Human Health. J Med Chem Toxicol, 2(1):40-46.

[15] Harris D, Pathan AK, Gothkar P, Joshi A, Chivasa W, Nyamudeza P. (2001). On-farm Seed priming: using participatory methods to revive and refine a key tecnology. Agricul Systems, 69(1-2), 151-164. 

http://dx.doi.org/10.1016/S0308-521X(01)00023-3.

[16] Inobeme A, Mathew J.T, Okonkwo S, Ajai A.I, Jacob J.O, Olori E. (2020). Pesticide residues in food: distribution, route of exposure and toxicity: in review. MOJ Food Process Technols., 8(3):121-124. DOI: 10.15406/mojfpt.2020.08.00251.

[17] Varma J and Dubey N.K. (1999). Prospectives of botanical and microbial products as pesticides of tomorrow. Curr Sci., 76:172-179.

[18] Iwuagwu C.C, Onejeme F.C, Ononuju C.C, Umechuruba C.I, Nwogbaga A.C. (2018). Effects of Plant Extracts and Synthetic Fungicides on the Radial Growth of Phoma oryzae on Rice (Oryza sativa L.) in Some Rice Growing Areas of South Eastern Nigeria. J Plant Pathol Microbiol., 9: 468. doi:10.4172/2157-7471.1000468.

[19] Adesina, S. K., C. O. Adewumi, and V. O. Marrquis. (1980). Phytochemical investigations of the molluscicidal properties of Tetrapleura tetraptera (Taub). J. Afr. Med. Plants., 3:7-15.

[20] Dosunmu, M. I. (1997). Chemical composition of the fruit of Tetrapleura tetraptera and The physicochemical properties of its oil. Global J. Pure Appl. Sci., 3(1): 61-67.

[21] Adesina S.K, Iwalewa E.O, and Johnny I.I. (2016). Aidan fruit (Tetrapleura tetraptera Taub) ethnopharmacology, chemistry, medicinal and nutritional values-A review). British Journal of Pharmaceutical Research, 12: 1-22.

[22] Barnett, H.L. and Hunter, B.B. (1998). Illustrated Genera of Imperfect Fungi, 4th Edition. APS Press.

[23] ISTA (International Seed Testing Association). (1985). International rules for seed testing. Seed Sci. Technol., 13: 299-355.

[24] Matsushima K and Sakagami J. (2013). Effects of seed hydropriming on germination and Seedling vigor during emergence of rice under different soil moisture conditions. Am J Plant Sci., 4:1584-1593.

[25] Association of Official Seed Analysts (AOSA). (1983). Seed vigor testing handbook. East Lansing: 93p. (Contribution 32).

[26] Dhanamanjuri, W, Thoudam, R, and Dutta, B.K. (2013). Effect of Some Pesticides (Fungicides) on the Germination and Growth of Seeds/Seedlings of Some Crop Plants, (i.e. Cicer arietinum and Zea mays). Middle-East Journal of Scientific Research, 17 (5): 627-632. DOI:10.5829/idosi.mejsr.2013.17.05.12221.

[27] Goulart, I., Matzenbacher, F., and Merotto, A. (2012). Differential germination pattern of rice cultivars resistant to imidazolinone herbicides carrying different acetolactate synthase gene mutations. Weed Research, 52(3).

[28] Serferbe S, G.R. Tsopmbeng Noumbo, and Kuiate Jules Roger. (2016). Seed-Borne Fungi Associated with Rice Seeds Varieties in Bongor, Chad Republic. Int. J. Curr. Microbiol. App. Sci., 5(12): 161-170.

[29] Iwuagwu, C.C., Ononuju, C.C., Umechuruba, C.I., Nwogbaga A.C., Obidiebub, A.E. Okolie H., Obasi C.C., and Uwaoma A.O. (2020). Effect of plant extracts on radial growth of helminthosporium oryzae causative of brown spot disease of rice under in-vitro. African Crop Science Journal, Vol. 28, No. 3, pp. 473-480. DOI: https://dx.doi.org/10.4314/acsj.v28i3.10.

[30] Singh, J., Bhatnagar, S. K., and Tomar, A. (2019). Study on fungicidal effect of plant extracts on plant pathogenic fungi and the economy of extract preparation and efficacy in comparison to synthetic/ chemical fungicides. Journal of Applied and Natural Science, 11(2): 333-337. https://doi.org/10.31018/jans. v11i2.2053.

[31] Anitha. S. R and Savitha, G. (2015). Impact of Mancozeb Stress on Seedling growth, Seed Germination, Chlorophyll and Phenolic Contents of Rice Cultivars. International Journal of Science and Research (IJSR): Volume 4, Issue 7, pp. 292-296.

How to cite this paper

Effects of Variety and Fungicides on Germination Percentage and Seedling Vigor of Rice (Oryza sativa L.) Seeds

How to cite this paper:  C. C. Iwuagwu, P. C. Ozofor, U. O. Aguwa, D. E. Iheaturu, O. A. Apalowo, M. E. Ejiofor, D. C. Iwu. (2023) Effects of Variety and Fungicides on Germination Percentage and Seedling Vigor of Rice (Oryza sativa L.) Seeds. International Journal of Food Science and Agriculture7(1), 85-94.

DOI: http://dx.doi.org/10.26855/ijfsa.2023.03.013