IJCEMR

Article 10.26855/ijcemr.2019.04.001

MiR-140-5p Targeted YES1 Inhibits Proliferation of Lung Cancer Cell A549

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Xiaoyu Wang

Chengde Medical College, An'yuan road, Shuangqiao District, Chengde City, Hebei province, 067000, China

Yanhong Yang

Qinhuangdao First Hospital, No. 258 Wenhua Road, Haigang District, Qinhuangdao city, Hebei province, 066000

Zhenzhen Wang

Qinhuangdao First Hospital, No. 258 Wenhua Road, Haigang District, Qinhuangdao city, Hebei province, 066000

Zishu Zhao

Chengde Medical College, An'yuan road, Shuangqiao District, Chengde City, Hebei province, 067000, China

Haixia wang

Hebei Medical University, Shijiazhuang Hebei Medical University, 050000, China

*Corresponding author: Yang yanhong, +8613223353870

180

Published: April 1,2019

Abstract

Objective: To test the expression difference of mir-140-5p between lung cancer tissues and lung tissues, and to observe whether miR-140-5p can regulate the expression of YES1, a proto-oncogene, so as to affect cell proliferation and apoptosis. This study provides research direction for the accumulation of data for targeted treatment of lung adenocarcinoma. Methods: The clinical information related to 45 NSCLC cancer cases was collected, and the expression differences of MiR-140-5p between tumor and para-carcinoma tissues was tested by qRT-PCR. After transfection for 48h, A549 cells were divided into a MiR-140-5p mimic group and a control group. Apoptosis of cells in each group was detected by flow cytometry. According to the predicted action sites of MiR-140-5p in YES1 from bioinformatics, whole-gene synthesis of 3’utr sequences containing target sites and mutant 3’utr sequences was performed, and dual-fluorescent vector detection was performed to investigate whether YES1 was the target gene of MiR-140-5p. Results: By comparing the expression of MiR-140-5p in tumor and para-carcinoma tissues by fluorescence PCR, it was found that the expression of MiR-140-5p in para-carcinoma tissues was much higher than that in tumor tissues (P < 0.05). Flow cytometry was used to detect the apoptosis of lung adenocarcinoma cells transfected with MiR-140-5p, and it was confirmed that MiR-140-5p could promote the apoptosis of lung cancer cells. According to the predicted action sites of MiR-140-5p in YES1 from bioinformatics, the 3’utr sequence containing the target sites and the mutant 3’utr sequence were synthesized, and the fluorescence reporter vector detection confirmed that YES1 was the target gene of MiR-140-5p.

Keywords

MiR-140-5p; Lung adenocarcinoma. A549 cell line; C

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How to cite this paper

MiR-140-5p Targeted YES1 Inhibits Proliferation of Lung Cancer Cell A549

How to cite this paper: Xiaoyu Wang, Yanhong Yang, Zhenzhen Wang, Zishu Zhao, Haixia wang. (2019) MiR-140-5p Targeted YES1 Inhibits Proliferation of Lung Cancer Cell A549. International Journal of Clinical and Experimental Medicine Research, 3(2), 100-105.

DOI: 10.26855/ijcemr.2019.04.001