Article http://dx.doi.org/10.26855/ijcemr.2023.10.031

Bibliometric Analysis of Epididymal Sperm Maturation Over the Past 20 Years


Jiaxin Zhang1,#, Qinhua Lei2,#, Xiaoyan Wang3, Sen Fu1, Xinyi Dong1, Yafei Kang1, Donghui Huang1,4,*

1Institute of Reproduction Health Research, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.

2Department of Human Anatomy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.

3Reproductive Center, Qingdao Women and Children's Hospital, Qingdao Women and Children's Hospital Affiliated to Qingdao Uni-versity, Qingdao, Shandong, China. 

4Shenzhen Huazhong University of Science and Technology Research Institute, Shenzhen, Guangdong, China.

#Both authors contributed equally to this paper.

*Corresponding author:  Donghui Huang

Published: November 30,2023


Background: The epididymis, as an important male sexual organ, plays a key role in sperm transport, maturation, and storage. In recent years, the quality of male semen has been declining, and the role of the epididymis in the process of sperm maturation has gradually attracted the attention of scholars, and there is no bibliometric analysis in this field. This study aimed to conduct a bibliometric analysis of research trends in the field of epididymal sperm maturation over the past 20 years. Methods: The articles and reviews related to sperm maturation in the epididymis from 2003 to 2022 were searched in the WOS core database. Vosviewers, Citespace, and the bibliometrix R software packages were used for bibliometric analysis. Results: We found 1102 articles published in English between January 2003 and December 2022. The number of publications related to epididymal sperm maturation is slowly fluctuating and rising. The United States and China are the two countries that have published the most articles in this field, while the United States and Australia are the two countries that have been most active in international cooperation. Newcastle University, Laval University, and McGill University are the main research institutions. Biological of Reproduction is the most popular journal in the field and the most cited journal. Nixon Brett has published the most papers and Sullivan Robert has been cited the most. Through cluster analysis, it was found that the main directions of this research field are androgen receptor, oxidative stress, sperm capacitation, basal cells, gamete biology, sperm development, epigenetics, proteomics, acrosome reaction, and transgenes. Extracellular vesicles, oxidative stress, male infertility, transcriptomics, epigenetics, and epididymal head, body, and tail are emerging and important research hotspots. Conclusion: This paper is the first bibliometric analysis in the field of epididymal sperm maturation. This article will provide the latest research directions and hot spots, which will provide a ref-erence for scholars studying the function of the epididymis.


[1] Agarwal A, Baskaran S, Parekh N, Cho CL, Henkel R, Vij S, Arafa M, Panner Selvam MK, Shah R: Male infertility. Lancet 2021, 397(10271):319-333.

[2] Levine H, Jørgensen N, Martino-Andrade A, Mendiola J, Weksler-Derri D, Jolles M, Pinotti R, Swan SH: Temporal trends in sperm count: a systematic review and meta-regression analysis of samples collected globally in the 20th and 21st centuries. Hum Reprod Update 2023, 29(2):157-176.

[3] Seymen CM: The other side of COVID-19 pandemic: Effects on male fertility. J Med Virol 2021, 93(3):1396-1402.

[4] Cornwall GA: New insights into epididymal biology and function. Hum Reprod Update 2009, 15(2):213-227.

[5] Johnston DS, Jelinsky SA, Bang HJ, DiCandeloro P, Wilson E, Kopf GS, Turner TT: The mouse epididymal transcriptome: transcriptional profiling of segmental gene expression in the epididymis. Biol Reprod 2005, 73(3):404-413.

[6] Sullivan R, Frenette G, Girouard J: Epididymosomes are involved in the acquisition of new sperm proteins during epididymal transit. Asian J Androl 2007, 9(4):483-491.

[7] Dacheux JL, Dacheux F: New insights into epididymal function in relation to sperm maturation. Reproduction 2014, 147(2):R27-42.

[8] Sharma U, Sun F, Conine CC, Reichholf B, Kukreja S, Herzog VA, Ameres SL, Rando OJ: Small RNAs Are Trafficked from the Epididymis to Developing Mammalian Sperm. Dev Cell 2018, 46(4):481-494.e486.

[9] Elbashir S, Magdi Y, Rashed A, Henkel R, Agarwal A: Epididymal contribution to male infertility: An overlooked problem. Andrologia 2021, 53(1):e13721.

[10] Ke L, Lu C, Shen R, Lu T, Ma B, Hua Y: Knowledge Mapping of Drug-Induced Liver Injury: A Scientometric Investigation (2010-2019). Front Pharmacol 2020, 11:842.

[11] van Eck NJ, Waltman L: Software survey: VOSviewer, a computer program for bibliometric mapping. Scientometrics 2010, 84(2):523-538.

[12] Chen C, Song M: Visualizing a field of research: A methodology of systematic scientometric reviews. PLoS One 2019, 14(10):e0223994.

[13] Aria M, Cuccurullo C: bibliometrix: An R-tool for comprehensive science mapping analysis. Journal of Informetrics 2017, 11(4):959-975.

[14] Sullivan R, Saez F, Girouard J, Frenette G: Role of exosomes in sperm maturation during the transit along the male reproductive tract. Blood Cells Mol Dis 2005, 35(1):1-10.

[15] Sullivan R, Saez F: Epididymosomes, prostasomes, and liposomes: their roles in mammalian male reproductive physiology. Reproduction 2013, 146(1):R21-35.

[16] Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227(5259):680-685.

[17] Jelinsky SA, Turner TT, Bang HJ, Finger JN, Solarz MK, Wilson E, Brown EL, Kopf GS, Johnston DS: The rat epididymal transcriptome: comparison of segmental gene expression in the rat and mouse epididymides. Biol Reprod 2007, 76(4):561-570.

[18] Dacheux JL, Gatti JL, Dacheux F: Contribution of epididymal secretory proteins for spermatozoa maturation. Microsc Res Tech 2003, 61(1):7-17.

[19] de Santi F, Beltrame FL, Hinton BT, Cerri PS, Sasso-Cerri E: Reduced levels of stromal sex hormone-binding globulin and an-drogen receptor dysfunction in the sperm storage region of the rat epididymis. Reproduction 2018, 155(6):467-479.

[20] Zhang FP, Malinen M, Mehmood A, Lehtiniemi T, Jääskeläinen T, Niskanen EA, Korhonen H, Laiho A, Elo LL, Ohlsson C et al: Lack of androgen receptor SUMOylation results in male infertility due to epididymal dysfunction. Nat Commun 2019, 10(1):777.

[21] Bhanmeechao C, Srisuwatanasagul S, Prapaiwan N, Ponglowhapan S: Reproductive aging in male dogs: The epididymal sperm defects and expression of androgen receptor in reproductive tissues. Theriogenology 2018, 108:74-80.

[22] Chen SR, Liu YX: Regulation of spermatogonial stem cell self-renewal and spermatocyte meiosis by Sertoli cell signaling. Repro-duction 2015, 149(4):R159-167.

[23] Chauvigné F, Parhi J, Ollé J, Cerdà J: Dual estrogenic regulation of the nuclear progestin receptor and spermatogonial renewal during gilthead seabream (Sparus aurata) spermatogenesis. Comp Biochem Physiol A Mol Integr Physiol 2017, 206:36-46.

[24] Wu PY, Scarlata E, O'Flaherty C: Long-Term Adverse Effects of Oxidative Stress on Rat Epididymis and Spermatozoa. Antioxidants (Basel) 2020, 9(2).

[25] Li Y, Zhao W, Fu R, Ma Z, Hu Y, Liu Y, Ding Z: Endoplasmic reticulum stress increases exosome biogenesis and packaging relevant to sperm maturation in response to oxidative stress in obese mice. Reprod Biol Endocrinol 2022, 20(1):161.

[26] Gibb Z, Blanco-Prieto O, Bucci D: The role of endogenous antioxidants in male animal fertility. Res Vet Sci 2021, 136:495-502.

[27] Baskaran S, Panner Selvam MK, Agarwal A: Exosomes of male reproduction. Adv Clin Chem 2020, 95:149-163.

[28] Chen H, Alves MBR, Belleannée C: Contribution of epididymal epithelial cell functions to sperm epigenetic changes and the health of progeny. Hum Reprod Update 2021, 28(1):51-66.

[29] Sharma U, Conine CC, Shea JM, Boskovic A, Derr AG, Bing XY, Belleannee C, Kucukural A, Serra RW, Sun F et al: Biogenesis and function of tRNA fragments during sperm maturation and fertilization in mammals. Science 2016, 351(6271):391-396.

[30] Wang H, Wang Z, Zhou T, Morris D, Chen S, Li M, Wang Y, Zheng H, Fu W, Yan W: Small RNA shuffling between murine sperm and their cytoplasmic droplets during epididymal maturation. Dev Cell 2023, 58(9):779-790.e774.

[31] Bedi YS, Roach AN, Thomas KN, Mehta NA, Golding MC: Chromatin alterations during the epididymal maturation of mouse sperm refine the paternally inherited epigenome. Epigenetics Chromatin 2022, 15(1):2.

How to cite this paper

Bibliometric Analysis of Epididymal Sperm Maturation Over the Past 20 Years

How to cite this paper: Jiaxin Zhang, Qinhua Lei, Xiaoyan Wang, Sen Fu, Xinyi Dong, Yafei Kang, Donghui Huang. (2023) Bibliometric Analysis of Epididymal Sperm Maturation Over the Past 20 YearsInternational Journal of Clinical and Experimental Medicine Research7(4), 686-695.

DOI: http://dx.doi.org/10.26855/ijcemr.2023.10.031