Article http://dx.doi.org/10.26855/ijfsa.2019.12.004

Variability analysis of the seeds and oil yields of several accessions Citrullus colocynthis (L.) collected in Morocco


BENMOUMOU Abdelhamid, EL MADIDI Saïd *

Biometrics and Bio Resources Laboratory Biotechnologies and Natural Resources Development (LBVRN) Faculty of Sciences, University Ibn Zohr, Agadir, Morocco.

*Corresponding author: EL MADIDI Saïd

Published: December 25,2019


The ripe fruits of Citrullus colocynthis (L.) Schrad were collected in 12 different localities in Morocco to evaluate the variability of seed and oil yields. The measured traits are: seed weight (SW), seed number (SN), weight of 100 seeds (W 100), seed oil content (OC), seeds yield (SY) and oil yield (OY). The seed oil content ranging between 17 and 24 % of seed weight. The extrapolated seed yield among the accessions ranged from 156 to 816 Kg ha-1, with an overall mean of 413 Kg ha-1. The oil yield varied between 35 to 172 Kg ha-1. The oil extracted from the seed revealed high levels of unsaturated fatty acids (74%) with C 18: 2 (60.06%) and C 18: 1 (11.14%). The results of anova (GLM) show significant and very highly significant effect saccording to the characters measured.

Significant variability was observed for seed and oil yields. This variability could be exploited for the selection of accessions with high oil yields.


[1] Si, Y., Dane, F., Rashotte, A., Kang, K., Singh, N. K. (2010). Cloning and expression analysis of the Ccrboh gene encoding respiratory burstoxidase in Citrullus colocynthis and grafting onto Citrulluslanatus (water melon). Exp Bot, 61 (6), 1635-1642

[2] Wang, Z., Hu, H., Goertzen, L.R., McElroy, J.S., Dane, F. (2014). Analysis of the Citrullus colocynthis Transcript omeduring Water Deficit Stress. PLoS ONE, 9(8), e104657

[3] Hassan, A., Younis, R. A., Rashed, M., El-domyati, F. M., and Rashed, N. A. (2016). molecular markers associated with droughttol-erance in Citrullus colocynthis. Egyptian Journal of Genetics And Cytology, 41(2).

[4] El Madidi, S., BenMoumou, A. andHakimi, F. (2017). Variability in the Response of Citrullus Colocynthis (L.) to Water Deficiency Stresses. In Euro-Mediterranean Conference for Environmental Integration. Springer, Cham, pp. 1361-1362.

[5] Verma, K. S., ul Haq, S., Kachhwaha, S. andKothari, S. L. (2017). RAPD and ISSR marker assessment of genetic diversity in Citrullus colocynthis (L.) Schrad: a unique source of germplasm highly adapted to drought and high-temperature stress. 3 Biotech, 7(5), 288.

[6] Si, Y., Zhang, C., Meng, S. and Dane, F. (2009). Gene expression changes in response to drought stress in Citrullus colocynthis. Plant cell reports, 28(6), 997-1009.

[7] Gurudeeban, S., Satyavani, K. and Ramanathan, T. (2010). Bitter apple (Citrullus colocynthis): An overview of chemical composition and biomedical potentials. Asian Journal of plant sciences, 9(7), 394.

[8] Marzouk, B., Marzouk, Z., Haloui, E., Fenina, N., Bouraoui, A., Aouni, M. (2010). Screening of analgesic and anti-inflammatory activities of Citrullus colocynthis from southern Tunisia. Journal of ethnopharmacology. 128 (1), 15-19.

[9] Pravin, B., Tushar, D., Vijay, P. and Kishanchnad, K. (2013). Review on Citrullus colocynthis. Int. J. Res. Pharm. Chem, 3(1), 46-53.

[10] Hussain, A. I., Rathore, H. A., Sattar, M. Z. A., Chatha, S. A. S., Sarker, S. D., Gilani A. H. (2014). Citrullus colocynthis (L.) Schrad (bitter apple fruit): A review of its phytochemistry, pharmacology, traditional uses and nutritional potential. J. Ethnopharmacol, 155, 54-66.

[11] Riaz, H., Chatha, S. A. S., Hussain, A. I., Bukhari, S. A., Hussain, S. M., & Zafar, K. (2015). Physico

chemicalcharacterization of bitter apple (Citrulluscolosynthis) seed oil and seed residue. Int. J. Biosci, 6(1), 283-292.

[12] Nehdi, I. A., Sbihi, H., Tan, C. P. and Al-Resayes, S. I. (2013). Evaluation and characterisation of Citrullus colocynthis (L.) Schrad seed oil: Comparison with Helianthusannuus (sunflower) seed oil. Food chemistry, 136(2), 348-353

[13] Giwa, S., Abdullah, L. C., Adam, N. M. (2010). Investigating “Egusi” (Citrullus colocynthis L.) seed oil as potential biodiesel feeds tock. Energies, 3(4), 607-618.

[14] Bello, E. and Makanju, A. (2011). Performance evaluation of Egunsi melon (Citrullus colocynthis L.) seedsoil biodiesel. Journal of Emerging Trends in Engineering and Applied Sciences, 2(5), 741-745.

[15] SAS Institute, (2010). Statistical analysis Software version 9.3 user's manual., Cary, NC: SAS Institute Inc. USA.

[16] Dane, F., Liu, J. and Zhang, C. (2007). Phylogeography of the bitter apple, Citrullus colocynthis. Genetic Resources and Crop Evolution, 54(2), 327-336.

[17] Chavan, S. B., Kumbhar, R. R. and Sharma, Y. C. (2014). Transesterification of Citrullus colocynthis (Thumba) oil: Optimization for biodiesel production. Adv Appl Sci Res, 5(3), 10-20.

[18] Giwa, S. O., Chuah, L. A. and Adam, N. M. (2014). Fuel properties and rheological behavior of biodiesel from egusi (Colocynthiscitrullus L.) seed kernel oil. Fuel Processing Technology, 122, 42-48.

[19] Menon, K., Sood, N., Rao, N. K., (2016). Study of morpho-agronomic diversity and oil content in desert gourd ('Citrullus colocynthis' (L.) Schrad.). Australian Journal of Crop Science, 10 (7), 1000.

[20] Sawaya, W. N., Daghir, N. J., Khan, P. (1983). Chemical characterization and edibility of the oilextracted from Citrullus colocynthis seeds. Journal of Food Science, 48(1), 104-106.

[21] Sadou, H., Sabo, H., Alma, M. M., Saadou, M., Leger, C. L. (2007). Chemical content of the seeds and physico-chemical characteristic of the seed oils from Citrullus colocynthis, Cocciniagrandis, Cucumismetuliferus and Cucumisprophetarum of Niger. Bulletin of the Chemical Society of Ethiopia, 21(3), 323-330.

[22] Mertia, R.S. and Gupta, I.C., (1994). Cultivate the colocynth in waste lands. Indian Horticulture, April-June, 1994, 40-41.

[23] Mahajan, S. S. and Kumawat, R. N., (2013). Study of seeddormancy in colocynth (Citrullus colocynthis L.) Withafter-ripening of fruits, seed extraction procedures and period of seedstorage. Natl. Acad. Sci. Lett. 36, 373-378.

How to cite this paper

Variability analysis of the seeds and oil yields of several accessions Citrullus colocynthis (L.) collected in Morocco

How to cite this paper: Abdelhamid, B., Saïd, E. M. (2019) Variability analysis of the seeds and oil yields of several accessions Citrullus colocynthis (L.) collected in Morocco. International Journal of Food Science and Agriculture, 3(4), 287-291.

DOI: http://dx.doi.org/10.26855/ijfsa.2019.12.004