Article http://dx.doi.org/10.26855/ijfsa.2020.03.012

A Review on “Bovine Sub-Clinical Mastitis in Nepal: Sustainable Management Strategy.”


A. Bhattarai 1,*, K. Kaphle 1, P. Adhikari 2

1 Institute of Agriculture and Animal Science, Paklihawa Campus, Tribhuvan University, Nepal.

2 Agriculture and Forestry University, Rampur, Chitwan, Nepal.

*Corresponding author: A. Bhattarai, Institute of Agriculture and Animal Science, Paklihawa Campus, Tribhuvan University, Nepal.

Published: March 12,2020


Nepalese bovine farming is crucial yet underperforming enterprise in Nepal. Inadequate husbandry, lack of proper nutrition and increasing cost of inputs in relationship with the price of outputs makes it a risky business. Diseases and reproductive pathologies tops the list and mastitis is one among top three threats found by farmers of Nepal. Sub-clinical mastitis is characterized by no visible changes in the appearance of the milk and udder but milk production decreases and if timely detected saves lots of trouble to the animal, farmer and the veterinarian. The aim of this paper is to review reported information on status, associated risks and preventive measures of sub-clinical mastitis in bovines of Nepal. A systematic review of available papers published in Nepalese and International magazines, journals, conference souvenirs through hard copy and online search from Google Scholar, Research Gate was done. The obtained information was thoroughly reviewed and the prevalence incidence rate, microbes involved, treatment approach adopted was reanalyzed and summarized. Sub clinical mastitis is serious problem from both farmers and consumers’ point of view. Given the era of antimicrobial resistance, consumer concerns, cost of treatment and stress of high production-sustainable management is likely the best approach. 


1. Bhanderi, B.M. & Garg, M.R. (2012). A study on reducing the incidence of subclinical and clinical mastitis in dairy cows by feeding vitamins and minerals based strategic feed supplement. Indian Journal of Dairy Science, 65 (5), 388-392.

2. Bindari, S. S. and Y. R. (2012). Prevalence of Sub-Clinical Mastitis among Dairy Cattle in Bhaktapur District, Nepal. International Journal of Agriculture and Biosciences, 1(1), 16–19.

3. Chakrabarti, A. (2014). A textbook of preventive veterinary medicine. Kalyani.

4. Contreras, G. A., & Rodríguez, J. M. (2011). Mastitis: comparative etiology and epidemiology. Journal of Mammary Gland Biology and Neoplasia, 16(4), 339–356. https://doi.org/10.1007/s10911-011-9234-0

5. Cressier, B., & Bissonnette, N. (2011). Assessment of an extraction protocol to detect the major mastitis-causing pathogens in bovine milk. Journal of Dairy Science. https://doi.org/10.3168/jds.2010-3669

6. Dhakal, I. P. (2006). Normal somatic cell count and subclinical mastitis in Murrah buffaloes. Journal of Veterinary Medicine Series B: Infectious Diseases and Veterinary Public Health, 53(2), 81–86. https://doi.org/10.1111/j.1439-0450.2006.00918.x

7. Dhakal, Ishwari Prasad, Dhakal, P., Koshihara, T., & Nagahata, H. (2007). Epidemiological and bacteriological survey of buffalo mastitis in Nepal. The Journal of Veterinary Medical Science, 69(12), 1241–1245. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/18176019

8. Dhakal, I. P., & Thapa, B. B. (2003). Economic impact of clinical mastitis in buffaloes during lactation. Nepalese Vet. J, 27, 24-33.

9. Dhakal, I. P., & Tiwari, K. R. (1992). Epidemiological investigation of subclinical mastitis in western Chitwan. Nepal. Vet. Review, 7, 6-10.

10. Dilshad, S. R., Rehman, N. U., Ahmad, N., & Iqbal, A. (2010). Documentation of ethnoveterinary practices for mastitis in dairy animals in Pakistan. Pakistan Veterinary Journal, 30(3), 167-171.

11. Dua, K. (2001). Incidence, etiology and estimated economic losses due to mastitis in Punjab and in India-An update. Indian dairyman, 53(10), 41-52.

12. Fagiolo, A., & Lai, O. (2007). Mastitis in buffalo. Italian Journal of Animal Science, 6(SUPPL. 2), 200–206. https://doi.org/10.4081/ijas.2007.s2.200

13. Galal, K., Hameed, A., Sender, G., & Korwin-kossakowska, A. (2007). Public health hazard due to mastitis in dairy cows *. 25(2), 73–85.

14. Horváth, G., Mohamed, A. I., Varga, J., Szemerédi, G., & Quarini, L. (1981). Effect of subclinical mastitis on milk composition. Acta Veterinaria Academiae Scientiarum Hungaricae, 29(3), 271–276. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/6753545

15. Islam, M., Islam, M., Islam, M., Rahman, M., & Islam, M. (2012). Prevalence of Subclinical Mastitis in Dairy Cows in Selected Areas of Bangladesh. Bangladesh Journal of Veterinary Medicine, 9(1), 73–78. https://doi.org/10.3329/bjvm.v9i1.11216

16. Jha, V. C., Thakur, R. P., Yadav, J. N., & Rai, L. B. (1993). Epidemiological investigation of subclinical bovine mastitis in the eastern hills of Nepal. Vet. Res. Kathmandu, 8(2), 35-39.

17. Joshi, H. D., & Joshi, B. R. (1997). Prevalence of Sub-clinical mastitis in cows and buffaloes in the western hills of Nepal. Veterinary Review, 12(1), 1-6.

18. Kabir, M., Ershaduzzaman, M., Giasuddin, M., Islam, M., Nazir, K., Islam, M., … Ali, M. (2017). Prevalence and identification of subclinical mastitis in cows at BLRI Regional Station, Sirajganj, Bangladesh. Journal of Advanced Veterinary and Animal Research, 4(3), 295. https://doi.org/10.5455/javar.2017.d227

19. Khakural, G. P. (1996). Study on prevalence of subclinical mastitis in Kathmandu Valley. In 1. National Workshon on Livestock/Fisheries Research in Nepal, Khumaltar, Lalitpur (Nepal), 7-9 May 1996. Communication, Publication and Documentation Division.

20. Khanal, T., & Pandit, A. (2013). Assessment of sub-clinical mastitis and its associated risk factors in dairy livestock of Lamjung, Nepal. International Journal of Infection and Microbiology, 2(2), 49–54. https://doi.org/10.3126/ijim.v2i2.8322

21. Lamsal, P. (2018). Cattle Hygiene Status and Its Relation with Subclinical Mastitis: A Study in Commercial Farms in Rampur, Nepal. International Journal of Applied Sciences and Biotechnology, 6(3), 252-254.

22. M, S., & HA, T. (2016). A Treatise on Bovine Mastitis: Disease and Disease Economics, Etiological Basis, Risk Factors, Impact on Human Health, Therapeutic Management, Prevention and Control Strategy. Advances in Dairy Research, 04(01), 1–10. https://doi.org/10.4172/2329-888x.1000150

23. Mn, B. N., Punniamurthy, N., Mekala, P., Ramakrishnan, N., & Sk, K. (2017). Ethno-veterinary Formulation for Treatment of Bovine Mastitis. 25–29.

24. Mooventhan, P., Manimaran, A., Kumar, R. S., Selvan, A. S., & Prakash, M. A. (2016). Indigenous ethnoveterinary medicinal practices for management of mastitis in dairy cattle. 50(1), 137–139. https://doi.org/10.18805/ijar.8568

25. Ottalwar Tanmay et al., O. T. et al. . (2017). Prevalence of Subclinical Mastitis in Buffaloes (Bubalus Bubalus) in Chhattisgarh, India. International Journal of Agricultural Science and Research, 8(1), 9–16. https://doi.org/10.24247/ijasrfeb20182

26. Pandit KS. Assessment of Sub-Clinical Mastitis using CMT, cultural examination and antibiogram in commercial dairy farms of Ratnanagar Municipality, Chitwan. B.V.Sc & A.H. Internship report (unpublished) submitted to Institute of Agriculture and Animal Science, Paklihawa Campus, 2018.

27. Patel, J. V, Bhingaradia, B. V, Patel, B. B., Patel, S. B., Patel, P. B., Vahora, S. P., … Division, E. (2012). Study on P r evalence of Mastitis and Antibiotic Pr Sensitivity of Bacterial Isolates R ecover ed from Recover ecovered Crossbred Cows of Anand District of Gujarat. 65(December), 467–471.

28. Priya, M. S., & Mohan, B. (2018). A front line demonstration on successful treatment of mastitis in dairy cows using ethno veterinary medicine. 7(1), 518–520.

29. Risco, C. A., Donovan, G. A., & Hernandez, J. (2010). Clinical Mastitis Associated with Abortion in Dairy Cows. Journal of Dairy Science, 82(8), 1684–1689. https://doi.org/10.3168/jds.s0022-0302(99)75397-x

30. Sharma N, GJ Rho, YH Hong, TY Kang, HK Lee, TY Hur and DK J, 2012. Bovine Mastitis: An Asian Perspective. Asian J of Ani and Vet Adv, 7: 454-476.

31. Shrestha, S., & Bindari, Y. R. (2012). Prevalence of sub-clinical mastitis among dairy cattle in Bhaktapur District, Nepal. International Journal of Agriculture and Biosciences, 1(1), 16-19.

32. Singh, K., & Pundir, R. S. (2001). Problems and prospects of smallholder dairy production and marketing in South Asia: An overview. In Proceedings of a South–South workshop held at National Dairy Development Board (NDDB) Anand, India (pp. 88-104).

33. Sinha, M. K., Thombare, N. N., & Mondal, B. (2014). Subclinical mastitis in dairy animals: Incidence, economics, and predisposing factors. Scientific World Journal, 2014. https://doi.org/10.1155/2014/523984

34. Subedi S. Assessment of Subclinical Mastitis using CMT, cultural examination & Antibiotic Sensitivity Test in dairy cattle of Bharatpur Metropoliton City, Chitwan. B.V.Sc & A.H. Internship report (unpublished) submitted to Institute of Agriculture and Animal Science, Paklihawa Campus, 2018.

35. Tiwari, J., Babra, C., Tiwari, H. K., Williams, V., Wet, S. De, Gibson, J., Mukkur, T. (2013). Vaccines & Vaccination Trends In Therapeutic and Prevention Strategies for Management of Bovine Mastitis : An Overview. 4(1), 1–11. https://doi.org/10.4172/2157-7560.1000176

36. Wolfenson, D., Leitner, G., & Lavon, Y. (2015). The disruptive effects of mastitis on reproduction and fertility in dairy cows. Italian Journal of Animal Science, 14(4), 650–654. https://doi.org/10.4081/ijas.2015.4125

37.  Yadav S and Deo SN (2010). Sub clinical bacterial mastitis (SCM) in cattle of eastern Terai of Nepal. Proceedings on 9th Conference of Nepal Veterinary Association, Veterinary Complex, Tripureswor, Kathmandu, Nepal, 2224 April, 2010, pp 5763.

How to cite this paper

A Review on “Bovine Sub-Clinical Mastitis in Nepal: Sustainable Management Strategy.”

How to cite this paper: Bhattarai, A., Kaphle, K., Adhikari, P. (2020) A Review on “Bovine Sub-Clinical Mastitis in Nepal: Sustainable Management Strategy.”. International Journal of Food Science and Agriculture, 4(1), 80-89.

DOI: http://dx.doi.org/10.26855/ijfsa.2020.03.012