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Study on Test Method for the Quantitation of Individual Sugar and Sugar Alcohol in Protein Powder by LC/MS/MS
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Abstract
Sugar and sugar alcohol were added to the health benefit product to give a favorite flavor of the products, but people have conditions that need sugar replacement such as sugar alcohol. Thus, a new LC/MS/MS method was developed to separate and quantify fructose, glucose, saccharose, maltose, lactose, and sorbitol in protein powder and tablet. With using of an MS detector with an ESI source, the mass information of the sugar and sugar alcohol components can be obtained along with the retention times, combination of chromatographic retention time and molecular mass weight improves the selectivity of sugar and sugar alcohol analysis. The linearity range of fructose is 2.13-83.26 μg/mL, sucrose is 2.05-80.12 μg/mL, glucose is 3.07-120.08 μg/mL, maltose is 7.26-283.41 μg/mL, lactose is 7.56-295.41 μg/mL, sorbitol is 1.98-77.42 μg/mL. The recoveries of each sugar component and sorbitol from protein powder product are 91%-110%. The matrix of protein powder, tablets, soft gel, and capsule with a matrix of soy protein isolate was validated, showing that the method is reliable and suitable for routine use.
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How to cite this paper
Study on Test Method for the Quantitation of Individual Sugar and Sugar Alcohol in Protein Powder by LC/MS/MS
How to cite this paper: Jinjing Li, Pengfei Hu, Fan Li, Yanjun Zhang, Peter Chang, Gary Swanson. (2024) Study on Test Method for the Quantitation of Individual Sugar and Sugar Alcohol in Protein Powder by LC/MS/MS. International Journal of Food Science and Agriculture, 8(1), 48-53.
DOI: http://dx.doi.org/10.26855/ijfsa.2024.03.007
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