IJCEMR
Network Pharmacology of Mongolian Medicine Ran Agar-8
TOTAL VIEWS: 761
Abstract
Objective: To further explore the relevant mechanism of action by using network pharmacology and animal experiments. Methods: To predict and screen the potential active ingredients of Mongolian medicine Ran agar-8 powder and its therapeutic targets for bronchial asthma using TCMSP, SwissTargetPredition, Batmant-TCM, GeneCards, OMIM, PharmGkb, TTD and Drug⁃ bank databases. STRING database was used for Protein-proteininteraction (PPI) analysis, and Metascape database was used for GO functional annotation and KEGG pathway enrichment analysis. Results: A total of 54 potentially active ingredients and 2862 disease targets of bronchial asthma were selected, and 181 common targets of bronchial asthma were predicted by veny diagram. Ten core targets including TNF, AKT1, IL6, TP53, IL1B, CASP3, BCL2, PTGS2, HIF1A and MMP9 were identified by PPI network analysis. 10 key components such as quercetin, Β -sitosterol, kaonaphthol, luteolin, stigsterol, meso-1, 4-bis- (4-hydroxy-3-methoxyphenyl) -2, 3-dimethylbutane, naringin, lupine palmitate, cystine, and baicalin were obtained through the "component-target" network analysis. Functional enrichment analysis showed that Ran agar-8 may play its role through cancer IL-17 signaling pathway, TNF signaling pathway, Th1 and Th2 cell differentiation, MAPK signaling pathway, NF-KB signaling pathway, TGF-β signal-ing pathway, and other signaling pathways. Conclusion: Through the network pharmacological analysis, the Mongolian medicine Ran agar-8 can play a role in the treatment of bronchial asthma through multi-components and multi-targets, so as to alleviate the bronchial remodeling process.
References
[1] Yu Tuo Yuandan Gongbu, compiled by the Institute of Mongolian Medicine of Inner Mongolia Autonomous Region. Four Medical Codes (Mongolian Edition) [M]. Hohhot: Inner Mongolia People's Publishing House, 1977:352.
[2] Bagena, Mongolian Medicine Formulae.[M]. Hohhot: Inner Mongolia People's Publishing House, 2007:150
[3] Ru J L, Li P, Wang J N, et al. TCMSP: a database of systems pharmacology for drug discovery from herbal medicines [J]. J Cheminform, 2014, 6:13.
[4] Gfeller D, Grosdidier A, Wirth M, et al. SwissTargetPrediction: a web server for target prediction of bioactive small molecules [J]. Nucleic Acids Res, 2014, 42:32-38.
[5] Liu Z Y, Guo F F, Wang Y, et al. BATMAN-TCM: a bioinformatics analysis tool for molecular mechanism of traditional Chinese medicine [J]. Sci Rep, 2016, 6:2146.
[6] Wang Z Y, Chu F H, Gu N N, et al. Integrated strategy of LC-MS and network pharmacology for predicting active constituents and pharmacological mechanisms of Ranunculus japonicus Thunb. for treating rheumatoid arthritis [J]. J Ethnopharmacol, 2021, 271:113818.
[7] Sangya P, Martin M J, O 'Donovan C. UniProt: the universal protein knowledgebase [J]. Nucleic Acids Res 2017, 45 (D1): D158-D169.
[8] Stelzer G, Rosen N, Plaschkes I, et al. The GeneCards suite: from gene data mining to disease genome sequence analyses [J].Curr Protoc Bioinformatics, 2016, 54:1301-1303.
[9] Amberger J S, Bocchini Ca, Schiettecatte F, et al. OMIM.org: Online Mendelian Inheritance in Man (OMIM (R)), an online catalog of human genes and genetic disorders [J]. Nucleic Acids Res, 2015, 43: D789-D798.
[10] Li G, Owen R P, Gor W, et al. Pharm GKB: an integrated resource of pharmacogenomic data and knowledge [J]. Curr Protoc Bioinformatics, 2008, 14:147.
[11] Zhou Y, Zhang Y T, Lian X C, et al. Therapeutic target database update 2022: facilitating drug discovery with enriched comparative data of targeted agents [J]. Nucleic Acids Res, 2022, 50 (D1): 398-1407.
[12] Wishart D S, Feunang Y D, Guo A C, et al. DrugBank 5.0: a major update to the DrugBank database for 2018 [J]. Nucleic Acids Res, 2018, 46 (D1): 1074-1082.
[13] Szklarczyk D, Gable A L, Lyon D, et al. STRING v11: protein-protein association networks with increased coverage, supporting functional discovery in genome-wide experimental datasets [J]. Nucleic Acids Res, 2019, 47 (D1): 607-613.
[14] Shannon P, Markiel A, Ozier O, et al. Cytoscape: a software environment for integrated models of biomolecular interaction networks [J]. Genome Res, 2003, 13 (11): 2498-2504.
[15] Zhou Y Y, Zhou B, Pache L, et al. Metascape provides a biologist-oriented resource for the analysis of systems-level datasets [J]. Nat Commun, 2019, 10 (1): 1523.
[16] Li yingge, Xiu Lan, Yin Long, et al. [23] Li Eng, Xiulan, et al. Clinical observation of 120 cases of bronchial asthma treated by Mongolian medicine Agaru-8 Powder [J]. Chinese Science and Technology of Traditional Chinese Medicine, 2014, 21 (1): 188-189.
How to cite this paper
Network Pharmacology of Mongolian Medicine Ran Agar-8
How to cite this paper: Yin Long, Ts Tuvshinjargal, Molor-Erdene Perenlei. (2024) Network Pharmacology of Mongolian Medicine Ran Agar-8. International Journal of Clinical and Experimental Medicine Research, 8(3), 442-449.
DOI: http://dx.doi.org/10.26855/ijcemr.2024.07.013
More Articles
- Regulation of Tumor-induced Platelet Clearance Modulated by the ERCC Family Through Mitochondrial Pathway
- A Review of Studies on the Effectiveness of Cognitive Behavioral Therapy in Treating Eating Disorders
- Clinical Application of Bilateral Proximal Lower Limb Tourniquet in the Emergency Treatment of Limb Fractures with Hemorrhagic Shock
- Application and Effect Evaluation of Orthopedic Surgery in Treating Adolescent Accessory Bone Pain
- Identification of the Alterations of Gut Microbiome and Its Relationship with NF-κB in Patients with Type 2 Diabetes and Diabetic Peripheral Neuropathy
- A Study of the Efficacy and Complications of Clavicular Hook Plates in the Treatment of Distal Clavicle Fractures