IJCEMR

Article http://dx.doi.org/10.26855/ijcemr.2020.10.009

Mechanism of Sanren Decoction in Treating Constipation Based on Network Pharmacology

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Zihua Guo 1,*, Yaping Cui 1, Chuan chuan Bi 2, Xia Xiao 2, Hua Jiang 2

1 Shaanxi University of Traditional Chinese Medicine, Xianyang, Shaanxi 712046, China.

2 Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine, Xianyang, Shaanxi 712000, China.

*Corresponding author: Zihua Guo

Published: September 10,2020

Abstract

Objective: To analyze the potential mechanism of Sanren Decoction in the treatment of constipation by using network pharmacological methods, and to provide a theoretical reference for further experimental research. Methods: The TCMSP, Uniprot, and Genecards databases were searched, and the targets of Sanren Tang chemical constituents and constipation-related targets were screened. Cytoscape software was used to construct the chemical composi-tion-target-disease network of Sanren Tang. The biological function and signal pathway were analyzed to explore the mechanism of Sanren Decoction in the treatment of constipation. Results: According to the screening conditions [oral bioavailability (OB) ≥ 30, drug-like (DL) ≥ 0.18)], a total of 502 active ingredients and 219 potential drug targets were obtained. A total of 2,842 disease targets were collected in the Genecards database and the UniProt database with “constipation” as the search condition 119 core target proteins of Sanren Decoction for constipation were screened, which mainly involved DRD1, CHRM3, CHRM1, PTGS2, RXRA and so on. The main biological processes of GO include: ubiquitin-like protein ligase binding, kinase-regulated activity, cytokine receptor binding, ubiquitin-protein ligase binding, and protein kinase regulator activity. KEGG enrichment results mainly include: PI3K-Akt signaling pathway, hepatitis B pathway, human cytomegalovirus infection pathway, hepatitis C pathway, Kaposi’s sarcoma-associated herpes virus infection pathway, and so on. Conclusion: The mechanism of Sanren Decoction in the treatment of constipation may be related to its multiple components acting on multiple targets, affecting multiple signal pathways related to constipation, and regulating complex biological processes.

References

[1] Jasper Pannemans, Karen Van den Houte, Benjamin Fischler, Hubert Piessevaux, Florencia Carbone, Jan Tack. (2020). Preva-lence and impact of self‐reported painful and non‐painful constipation in the general popu lation [J]. Neurogastroenterology & Motility, 2020, 32(4).

[2] Ye Mengyi, Niu Yang. (2017). Clinical Application of Sanren Decoction Bright Chinese Medicine, 2017, 32(06): 887-890.

[3] Wang Chengchuan, Liu Qiang, Peng Qifeng, Wang Yirui, Yan translated Xi, he Ping. (2018). An Analysis of Professor He Ping’s Experience in Treating Dehumidification and Heat Stagnation Constipation Based on Syndrome Differentiation of Sanjiao [J]. Sichuan TCM, 2018, 36(06): 7-9.

[4] Dou Xibin, Tang Hanqing. (2019). Application of Sanren Decoction [J]. Journal of Guangxi University of Traditional Chinese Medicine, 2019, 22(02): 38-40.

[5] Guo Dongfang, Luo Wenzhao, Zhang Weidong, Sun Yuxin. (2008). Application of San Ren Tang by Prof. Sun Yuxin [J]. New Chinese Medicine, 2008(05): 115.

[6] Duan Qi, Jiang An, Li Zhipeng, Yang Dong, He Ping. (2007). Add or subtract Sanren Decoction to treat 35 cases of dampness and heat accumulation of spleen constipation [J]. Colorectal Anal Surgery, 2007(04): 245-246.

[7] Zhang Huamin, Liu Sihong, Gao Hongjie, Gong Gupta Lei. (2019). Advances in Pharmacological Methods of Compound Tra-ditional Chinese Medicine Network [J]. Evaluation and Analysis of Drug Use in Chinese Hospitals, 2019, 19(10): 1270-1276.

[8] Jinlong Ru, Peng Li, Jinan Wang, Wei Zhou, Bohui Li, Chao Huang, Pidong Li, Zihu Guo, Weiyang Tao, Yinfeng Yang, Xue Xu, Yan Li, Yonghua Wang, Ling Yang. (2014). TCMSP: a database of systems pharmacology for drug disc overy from herbal medicines [J]. Springer International Publishing, 2014, 6(1).

[9] Liu Zhongyang, Guo Feifei, Wang Yong, Li Chun, Zhang Xinlei, Li Honglei, Diao Lihong, Gu Jiangyong, Wang Wei, Li Dong, He Fuchu. (2016). BATMAN-TCM: a Bioinformatics Analysis Tool for Molecular mechanism of Traditional Chinese Medicine [J]. Pubmed, 2016, 6.

[10] Luo Jingchu. (2019). UniProt Protein Database [J]. Bioinformatics, 2019, 17(03): 131-144.

[11] Fichilevich Simon, Noodles Ron, Rapa Portnoya, Hadar Red, Prasquez Inbar, Inestantztipi, Rosennaomi, Cohen Asher, Twik Michal, Saffron Marilyn, Lancet Dolon, Kendana. (2017). GeneHancer: genome-wide integration on of enhancers and target genes in GeneCards [J]. Pubmed, 2017.

[12] Holmås Stian, Puig Rafel Riudavets, Acencio Marcio Luis, Mironov Vladimir, Kuiper Martin. (2019). The Cytoscape BioGa-teway App: explorative network building from the BioGateway triple store [J]. Pubmed, 2019.

[13] Amit Kumar Subudhi, Pon Arunachalam Boopathi, Isha Pandey, Ramandeep Kaur, Sheetal Middha, Jyoti Acharya, Sanjay K. Kochar, Dhanpat K. Kochar, Ashis Das. (2015). Disease specific modules and hub genes for interv ention strtegies: A co-expression network based approach for Plasmodium falciparum clinical isolates [J]. Infection, Genetics and Evolution, 2015, 35.

[14] Yu Tang, Min Li, Jianxin Wang,Yi Pan, Fang-Xiang Wu. (2015). CytoNCA: A cytoscape plugin for centrality analysis and evaluation of protein interaction networks [J]. BioSystems, 2015, 127.

[15] Zong Yang, Sun Mingming, Yue Yinzi, Yan Shuai. (2018). A study on the mechanism of Atractylodes macrocephala-Fructus Aurantii in the treatment of chronic transit constipation based on network pharmacology [J]. China Pharmacy, 2018, 29(13): 1798-1802.

[16] Shi XZ, Lin YM, Powell DW, et al. (2011). Pathophysiology of motility dysfunction in bowel obstruction: role of stretch-induced COX-2 [J]. Am J Physiol Gastrointest Liver Physiol, 2011, 300(1): G99-G108.

[17] Li Jia. (2016). Role of PI3K/AKT/GSK3-b signal transduction pathway in salidroside protection of myocardial ische-mia-reperfusion injury in rats [J]. Journal of Cardiovascular and Cerebrovascular Diseases, 2016, 14(22): 2621-2624.

[18] Shahad W. Kattan, Mohamed S. Nafie, Gamal A. Elmgeed, Walla Alelwani, Muhammad Badar, Mohamed A. Tantawy. (2020). Molecular docking, anti-proliferative activity and induction of apoptosis in human liver cance r cells treated with androstane derivatives: Implication of PI3K/AKT/mTOR pathway [J]. Elsevier Ltd, 2020, 198.

[19] Zhang Huamin, Liu Sihong, Gao Hongjie, Gong Gupta Lei. (2019). Advances in Pharmacological Methods of Compound Tra-ditional Chinese Medicine Network [J]. Evaluation and Analysis of Drug Use in Chinese Hospitals, 2019, 19(10): 1270-1276.

[20] Liu Hong, Zhang Lingyan, Xue Yang. (2019). Experimental study of Xuefu Zhuyu decoction to relieve myocardial ische-mia-reperfusion injury in rats through PI3K/Akt pathway [J]. Pharmacology and Clinical Medicine, 2019, 35(05): 11-15.

[21] Li Lei, Gao Yizhao, Ma Lianping, Miao Cheng, Yang Hui. (2019). Asiaticoside attenuates brain tissue damage and induces angiogenesis in focal cerebral infarction rats by activating PI3K/Akt signaling pathway [J]. Chinese Journal of Immunology, 2019, 35(17): 2068-2078.

[22] Zhu Jing, Song Yue. (2019). Mechanism of low dose LBH589 inducing apoptosis of epithelial ovarian cancer cells through PI3K/AKT pathway [J]. Journal of Bengbu Medical College, 2019, 44(06): 708-711.

[23] Deng Heng. (2018). Study on c-kie mRNA of colonic tissue and PI3K/AKT/eNOS signaling pathway in rats with slow transit constipation [D]. Anhui University of Traditional Chinese Medicine.

[24] Li Minhao, Zhang Guozhi, Yuan Mei, Li Shuguang, Chen Junmao. (2015). The expression changes and significance of PI3K/AKT/eNOS signaling pathway related markers in gastrointestinal tissues of slow-transmission constipation rats [J]. Shandong Medicine, 2015, 55(19): 27-29.

[25] Li Minhao. (2015). Role of PI3K/AKT/eNOS signaling pathway in chronic transport constipation in rats [D]. North China University of Technology.

How to cite this paper

Mechanism of Sanren Decoction in Treating Constipation Based on Network Pharmacology

How to cite this paper: Zihua Guo, Yaping Cui, Chuan chuan Bi, Xia Xiao, Hua Jiang. (2020) Mechanism of Sanren Decoction in Treating Constipation Based on Network Pharmacology. International Journal of Clinical and Experimental Medicine Research, 4(4), 175-181.

DOI: http://dx.doi.org/10.26855/ijcemr.2020.10.009