IJCEMR
Identification of the Alterations of Gut Microbiome and Its Relationship with NF-κB in Patients with Type 2 Diabetes and Diabetic Peripheral Neuropathy
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Abstract
The surge in diabetic peripheral neuropathy (DPN) cases in recent years has underscored the urgent need for more intensive research into its prevention and management. The nuclear factor (NF)-κB is recognized as a key mediator in the development of diabetic microvascular complications, with alterations in gut microbiota dynamics being a significant factor in triggering NF-κB. Therefore, we proposed that there is a relationship among DPN, NF-κB, and gut microbiota. We conducted 16S rRNA sequencing to analyze the gut microbiota of individuals with DPN and type 2 diabetes (T2DM), and healthy controls, while also measured NF-κB levels. Our research findings indicate significant differences in the microbial composition among the three groups, particularly at the phylum level, with the control group showing a higher abundance of Firmicutes and a lower abundance of Bacteroidetes compared to the other two groups. Additionally, the α and β diversity were significantly lower in T2DM and DPN patients. Furthermore, a strong correlation was observed, indicating that the Clostridiales and Trichospirillaceae were significantly associated with levels of TG, FPG, and HDL-C. Moreover, specific gut bacteria, such as Coccosia, Barnesiella, and Roseburia, demonstrated robust associations with NF-κB level, suggesting the potential role in the pathophys-iology of DPN. Individuals afflicted with T2DM and DPN present with pro-nounced dysbiosis of the gut microbiota. This gut microbiota exhibits signifi-cant correlations with key physiological markers and clinical manifestations within the affected individuals, suggesting that these microbial shifts are inte-gral to the pathogenesis and progression of diabetic complications.
References
[1] Y. Wang, X. Ye, D. Ding, et al. Characteristics of the intestinal flora in patients with peripheral neuropathy associated with type 2 diabetes. J Int Med Res. 2020;48:300060520936806.
[2] Z. Zhou, B. Sun, D. Yu, et al. Gut Microbiota: An Important Player in Type 2 Diabetes Mellitus. Frontiers in cellular and infection microbiology. 2022;12:834485.
[3] J. Li, X. Hu, F. Liang, et al. Therapeutic effects of moxibustion simultaneously targeting Nrf2 and NF-κB in diabetic peripheral neuropathy. Applied biochemistry and biotechnology. 2019;189:1167-1182.
[4] T. Palacios, L. Vitetta, S. Coulson, et al. Targeting the Intestinal Microbiota to Prevent Type 2 Diabetes and Enhance the Effect of Metformin on Glycaemia: A Randomised Controlled Pilot Study. Nutrients. 2020;12.
[5] Y. Shen, X. Yu, Q. Wang, et al. Association between primary Sjögren's syndrome and gut microbiota disruption: a systematic review and meta-analysis. Clinical rheumatology. 2024;43:603-619.
[6] R. Polito, G. Costabile, E. Nigro, et al. Nutritional factors influencing plasma adiponectin levels: results from a randomised controlled study with whole-grain cereals. International journal of food sciences and nutrition. 2020;71:509-515.
[7] J. Yang, X. Yang, G. Wu, et al. Gut microbiota modulate distal symmetric polyneuropathy in patients with diabetes. Cell Metab. 2023;35:1548-1562 e1547.
[8] B.M. Duggan, A.K. Tamrakar, N.G. Barra, et al. Gut microbiota-based vaccination engages innate immunity to improve blood glucose control in obese mice. Molecular metabolism. 2022;55:101404.
[9] S. Sharma, P. Tripathi. Gut microbiome and type 2 diabetes: where we are and where to go? The Journal of nutritional biochemistry. 2019;63:101-108.
[10] J. She, G. Tuerhongjiang, M. Guo, et al. Statins aggravate insulin resistance through reduced blood glucagon-like peptide-1 levels in a microbiota-dependent manner, Cell Metab. 2024;36:408-421.e405.
[11] N. Priyadarsini, M. Ramachandran, K.K. Behera, et al. Association of serum NF-κB levels with peripheral neuropathy in type 2 diabetes mellitus patients: a pilot study. Hormone molecular biology and clinical investigation. 2024;45:27-33.
[12] H. Yang, T. Xie, D. Li, et al. Tim-3 aggravates podocyte injury in diabetic nephropathy by promoting macrophage activation via the NF-κB/TNF-α pathway. Molecular metabolism. 2019;23:24-36.
[13] M. Karin, Y. Yamamoto, Q.M. Wang. The IKK NF-kappa B system: a treasure trove for drug development, Nature reviews. Drug discovery. 2004;3:17-26.
[14] B.L. He, Q.W. Zheng, L.Q. Guo, et al. Structural characterization and immune-enhancing activity of a novel high-molecular-weight polysaccharide from Cordyceps militaris, International journal of biological macromolecules. 2020;145:11-20.
[15] X. Qin, Z. Liu, K. Nong, et al. Porcine-derived antimicrobial peptide PR39 alleviates DSS-induced colitis via the NF-κB/MAPK pathway. International immunopharmacology. 2024;127:111385.
[16] Z. Wang, J. Liu, F. Li, et al. Mechanisms of Qingyi Decoction in Severe Acute Pancreatitis-Associated Acute Lung Injury via Gut Microbiota: Targeting the Short-Chain Fatty Acids-Mediated AMPK/NF-κB/NLRP3 Pathway. Microbiology spectrum. 2023;11:e0366422.
[17] N.K. Pinna, R.M. Anjana, S. Saxena, et al. Trans-ethnic gut microbial signatures of prediabetic subjects from India and Denmark. Genome Medicine. 2021;13:36.
[18] H. Zhong, H. Ren, Y. Lu, et al. Distinct gut metagenomics and metaproteomics signatures in prediabetics and treatment-naïve type 2 diabetics. EBioMedicine. 2019;47:373-383.
How to cite this paper
Identification of the Alterations of Gut Microbiome and Its Relationship with NF-κB in Patients with Type 2 Diabetes and Diabetic Peripheral Neuropathy
How to cite this paper: Ruiling Zhao, Qian Qian, Qifan Zhu, Jin Wang. (2024) Identification of the Alterations of Gut Microbiome and Its Relationship with NF-κB in Patients with Type 2 Diabetes and Diabetic Peripheral Neuropathy. International Journal of Clinical and Experimental Medicine Research, 8(4), 640-645.
DOI: https://dx.doi.org/10.26855/ijcemr.2024.10.017
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