IJCEMR

Article http://dx.doi.org/10.26855/ijcemr.2021.07.014

Action of Fractionated Moringa oleifera Lam Leaf Extracts on Multidrug Resistant Pseudomonas aeruginosa Strains

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Eremwanarue Aibuedefe Osagie1,3,*, Erhauyi Osayemwenre2, Nwawuba Stanley Udogadi4, Shittu Hakeem Olalekan1

1Department of Plant Biology and Biotechnology, University of Benin, PMB, 1090, Ugbowo, Benin City, Nigeria. 

2Department of Pharmaceutical Chemistry, University of Benin, PMB, 1090, Ugbowo, Benin City, Nigeria. 

3Lahor Research Laboratories and Diagnostics Centre, 121, Old Benin-Agbor Road, Benin City, Nigeria. 

4Centre for Forensic Programmes and DNA Studies, University of Benin, PMB, 1090, Ugbowo, Benin City, Nigeria.

*Corresponding author: Eremwanarue Aibuedefe Osagie

Published: June 23,2021

Abstract

Pseudomonas aeruginosa is an opportunistic pathogen with the potential to cause serious surgical wound infections and remains a major problem especially in developing countries of the world. This has led to investigating antimicrobial actions of fractionated Moringa oleifera leaf extract on multidrug Pseudomonas aeruginosa strains. Isolation of P. aeruginosa strains from post-surgical wound in two locations used for the study, antibiotics susceptibility testing, qualitative and quantitative phytochemical screening were carried out using standard procedures followed by antibacterial testing of the different M. oleifera leaf extracts on selected multidrug resistant isolates. The results showed that 99 (35%) of patients studied had wound infection out of the total 284 samples collected. Thirty-four (54.8%) P. aeruginosa strains were found to show multidrug resistant ability from both locations studied. Qualitative analysis of phytoconstituents revealed the presence of flavonoid, phenol, saponins, steriods, tannin and terpenoids. Quantitative analysis showed that the different phytochemical recorded varying amount. Antibacterial assay results revealed that M. oleifera leaf n-hexane extract exhibited highest antibacterial activity against P. aeruginosa strain NAPCC-1 followed by methanol and aqueous methanol all at a concentration of 100 mg/ml while aqueous methanol best inhibited P. aeruginosa strain KAR12 growth followed by methanol then n-hexane extract. The varying effect could be attributed to the presence of phytochemicals in the different M. oleifera leaf extracts. The result of this study has shown the potentials of M. oleifera extracts as antibacterial agent by inhibiting the growth of the test organisms isolated from post-surgical wound infection.

Keywords

Pseudomonas aeruginosa, Moringa oleifera, wound swabs, Nosocomial infections

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How to cite this paper

Action of Fractionated Moringa oleifera Lam Leaf Extracts on Multidrug Resistant  Pseudomonas aeruginosa Strains

How to cite this paper: Eremwanarue Aibuedefe Osagie, Erhauyi Osayemwenre, Nwawuba Stanley Udogadi, Shittu Hakeem Olalekan. (2021) Action of Fractionated Moringa oleifera Lam Leaf Extracts on Multidrug Resistant Pseudomonas aeruginosa Strains. International Journal of Clinical and Experimental Medicine Research5(3), 316-329.

DOI: http://dx.doi.org/10.26855/ijcemr.2021.07.014