Article http://dx.doi.org/10.26855/ijfsa.2021.12.019

Development and Characterisation of Kainth Fruit Beverage Formulations: Nutritional Composition, Analysis, and Stability Study during Storage


Om Prakash1, Attar Singh Chauhan1, Ashok Kumar Maurya2, Vithal Balavant Kudachikar1,*

1Department of Fruit and Vegetable Technology, Academy of Scientific and Innovative Research (AcSIR), CSIR-Central Food Technological Research Institute, Mysuru, South-Central Karnataka state, India.

2National Dope Testing Laboratory, New Delhi-110003, India.

*Corresponding author: Vithal Balavant Kudachikar

Published: December 9,2021


This study aims to develop and characterize the ready-to-serve beverage formulations from enzyme liquefied Kainth fruit pulp and to assess their stability during storage. Xanthan gum showed the highest acceptability in terms of stability (zeta potential -29.10 mV) and sensory quality attributes, also carried out the effect of high-speed homogenization (20 MPa) on ready to serve beverages prepared with added xanthan gum. Both ready to serve beverage variations (with or without homogenization) showed high antioxidant potential (phenolics, flavonoids, and antioxidant activities) with better retention of vital nutrients (vitamins and minerals), organoleptically acceptable, microbiologically safe during storage, with shelf stability of these products up to four months at RT (29±2°C) and five months at LT (4±2°C) respectively. This study demonstrates that the product formulations from Kainth fruit have 4-6 months of shelf stability with a potential source of high phytonutrients and antioxidant properties that could promote and benefit human health.


[1] Ahmad, Z. (2007). Country report on plant genetic resources for food and agriculture. Pakistan Agricultural Research Council, Islamabad, Pakistan.

[2] Parmar, C. and Kaushal, M. K. (1982). Pyrus Pashia. In: Wild Fruits. India: Kalyani Publishers, New Delhi, pp. 78-80.

[3] Chettri, N., Sharma, E., and Lama, S. D. (2005). The non-timber forest produces utilization, distribution, and status in a trekking corridor of Sikkim, India. Lyonia, 8, 89-100.

[4] Siddiqui, S. Z., Ali, S., Rehman, A. U., Rubab, K., Abbasi, M. A., Ajaib, M., and Rasool, Z. G. (2015). Pyrus pashia, A persuasive source of natural antioxidants. Pakistan J Pharm Sci., 28, 1763-1772.

[5] Murad, W., Ahmed, A., Gilani, S. A., and Khan, M. A. (2011). Indigenous knowledge and folk use of medicinal plants by the tribal communities of Hazar Nao Forest, Malak, and District, North Pakistan. J Med Plants Res., 5, 1072-1086.

[6] Adebayo-Oyetoro, A. O., Ogundipe, O. O., Adeyemo, I. G., Ogundipe, F. O., Bamidele, F. A., and Adeyeye, S. A. O. (2016). Chemical, sensory, and shelf life study of pawpaw juice-milk blend. Cog Food Agri., 2, 1271-1276.

[7] Chauhan, A. S., Gul Afroze, S., Ramesh, M. N., Yadav, A. R., Rekha, M. N., and Ramteke, R. S. (2004). Optimization of papaya’s enzymatic liquefaction (Carica papaya L.) and jackfruit (Artocarpus heterophyllus Lam.) pulp using response surface methodology. Food Agri Environ, 2, 108-113.

[8] Ranganna, S. (1999) Handbook of analysis and quality control for fruits and vegetable products. 3rd Edition, Tata McGraw-Hill Publishing Co Ltd, New Delhi., India.

[9] Sami, R., Li, Y., Qi, B., Wang, S., Zhang, Q., Han, F., Ma, Y., Jing, J., and Jiang, L. (2014). HPLC Analysis of Water-Soluble Vitamins (B2, B3, B6, B12, and C) and Fat-Soluble Vitamins (E, K, D, A, and β-Carotene) of Okra (Abelmoschus esculentus). J Chem., 2014, 1-6. https://doi.org/10.1155/2014/831357.

[10] Singleton, V. L. and Rossi, J. A. (1965). Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. American J Enol Viticulture, 16, 144-158.

[11] Aadil, R. M., Zeng, X. A., Han, Z., and Sun, D. W. (2013). Effects of ultrasound treatments on quality of grapefruit juice. Food Chem., 141, 3201-3206.

[12] Wang, X., Beckhman, T.H., Morris, J.C., Chen, F., and Gangemi, J. D. (2008). Bioactivities of gossypol, 6-methoxygossypol, and 6, 6’-dimethoxygossypol. J Agri Food Chem., 56, 4393-4398.

[13] Nile, S. H. and Park, S. W. (2014). HPTLC analysis antioxidant, anti-inflammatory, and antiproliferative activities of Arisaema tortuosum tuber extract. Pharm Biol, 52, 221-227.

[14] Nile, S. H. and Park, S. W. (2014). Antioxidant: a-glucosidase and xanthine oxidase inhibitory activity of bioactive compounds from maize (Zea mays L.). Chem Biol Drug Des., 83, 119-125.

[15] Perestrelo, R., Silva, C., Silva, P., Medina, S. and Câmara, J. S. (2019). Differentiation of fresh and processed fruit juices using volatile composition. Molecules, 24, 974.

[16] Jennings, W. and Shibamoto, T. (1980). Qualitative Analysis of Flavor and Fragrance Volatiles by Gas Capillary Chromatography. USA: Academic Press, New York, 248-250.

[17] Anonymous. (2020). www.wildflavors.com/EMEA-EN/company/about-us.

[18] Adams, R. P. (2001). Identification of Essential Oil Components by Gas Chromatography/Quadrupole Mass Spectroscopy. Allured Publishing Corp, Carol Stream, IL, USA.

[19] Hemalatha, R., Kumar, A., Prakash, O., Supriya, A., Chauhan, A. S., and Kudachikar, V. B. (2018). Development and quality evaluation of ready-to-serve (RTS) beverage from Cape gooseberry (PhysalisperuvianaL.). Beverages, 4, 42.

[20] Bryant, C. M. and McClements, D. J. (2000). Optimizing preparation conditions for heat-denatured whey protein solutions to be used as cold-gelling ingredients. J Food Sci., 65, 259-263.

[21] Oziyci, H. R., Karhan, M., Tetik, N., and Turhan, I. (2012). Effects of processing method and storage temperature on clear pomegranate juice turbidity and color. J Food Process Pres., 37, 899-906.

[22] Ottaway, P. B. (2019). Fortification of beverages with vitamins and minerals. In: Functional and specialty beverage technology, 1st Edition. CRC Press, Washington, DC., USA, 71-91.

[23] Paine-Wilson, B. and Chen, T. S. (1979). Thermal destruction of folacin, the effect of pH and buffer ions. J Food Sci., 44, 717-722.

[24] Sharma, M., Gehlot, R., Singh, R., and Siddiqui, S. (2012). Changes in chemical constituents and overall acceptability of guava Jamun blend RTS drink and squash during storage. Beverage Food World, 39, 39-42.

[25] Yu, Y., Xu, Y., Wu, J., Xiao, G., Fu, M., and Zhang, Y. (2014). Effect of ultra-high pressure homogenization processing on phenolic compounds, antioxidant capacity and anti-glucosidase of mulberry juice. Food Chem., 153, 114-120.

[26] Cao, X., Bi, X., Huang, W., Wu, J., Hu, X., and Liao, X. (2012). Changes of quality of high hydrostatic pressure processed cloudy and clear strawberry juices during storage. Innov Food Sci Emerg Technol., 16, 181-190.

[27] Pettitt, D. J., Wayne, J. E. B., Nantz, J. J. R., and Shoemaker, C. F. (1995). Rheological Properties of Solutions and Emulsions Stabilized with Xanthan Gum and Propylene Glycol Alginate. J Food Sci., 60, 528-531.

[28] Zhou, L., Guan, Y., Bi, J., Liu, X., Yi, J., Chen, Q., Wu, X., and Zhou, M. (2017). Change of the rheological properties of mango juice by high-pressure homogenization. LWT-Food Sci Tech, 82, 121-130.

[29] Pang born, R. M., Gibbs, Z. M., and Tassan, C. (1978). Effect of hydrocolloids on apparent viscosity and sensory properties of selected beverages. J texture stud., 9, 415-436.

[30] Morrison, I. D. and Ross, S. (2012). Colloidal Dispersions. 6th Edition, APA, Wiley-Inter science, New York., USA.

[31] Yu, Z. Y., Jiang, S. W., Cao, X. M., Jiang, S. T., and Pan, L. J. (2016). Effect of high-pressure homogenization (HPH) on the physical properties of taro (Colocasia esculenta L). Schott) pulp. J Food Eng, 177, 1-8.

[32] Food Safety and Standards Authority of India. (2017). Standards for Non-Carbonated water-based beverages (Non-alcoholic) (Ref. No: Stds / SP (Water and Beverages). FDA Bhawan, Kotla Road, New Delhi, India.

How to cite this paper

Development and Characterisation of Kainth Fruit Beverage Formulations: Nutritional Composition, Analysis, and Stability Study during Storage

How to cite this paper: Om Prakash, Attar Singh Chauhan, Ashok Kumar Maurya, Vithal Balavant Kudachikar. (2021) Development and Characterisation of Kainth Fruit Beverage Formulations: Nutritional Composition, Analysis, and Stability Study during Storage. International Journal of Food Science and Agriculture5(4), 704-716.

DOI: http://dx.doi.org/10.26855/ijfsa.2021.12.019