This paper established a rapid, simple method for determination of absolute content of cyetpyrafen by qNMR. 1H NMR spectra was acquired with deuterodimethyl sulfoxide (DMSO-D6) as the solvent and hydroquinone as internal reference standard under the conditions of temperature 25℃, pulse angle 90°, pulses width 8.0 μs, relaxation delay 30 s, and scans 8. The proton peaks of cyetpyrafen (δ=6.35) and hydroquinone (δ=6.55) were taken as quantitative peaks. The peak area ratio y (As/Ar) and concentration ratio x (Cs/Cr) were linearly regressed, and the correlation coefficient was 0.9999. The RSD value of repeatability test was 0.80%, and the RSD value of stability test was 0.54%. The content of cyetpyrafen was determined as 99.8%. The result shows that qNMR can be used for quantitative determination of cyetpyrafen without reference standard, which is rapid, accurate and simple.
 Dusengem, S. L. and Zhang, J. Y. (2017). Advance on Method for Determination of Imidacloprid Pesticides Residues in vegetables. The Journal of the Science of Food and Agriculture, 1(1), 1-3.
 Bin Li, Haibo Yu, Yanmei Luo, et al. (2016). The Synthesis and Acaricidal Activity of SYP-9625. Modern Agrochemicals, 2016, 15(06): 15-16, 20.
 Jingyu Sun, Chunji Li, Jinqi Jiang, et al. (2022). Cross resistance, inheritance and fitness advantage of cyetpyrafen resistance in two-spotted spider mite, Tetranychus urticae. Pesticide Biochemistry and Physiology, 2022,105062. ISSN 0048-3575.
 Yanming Ye. (2017). Analysis of SYP-9625 TC by HPLC. Agrochemicals, 2017, 56(6): 424-426.
 Simmler, C., Napolitano, J. G., McAlpine, J. B., et al. (2014). Universal quantitative NMR analysis of complex natural samples. Current Opinion in Biotechnology, 2014, 25: 51-59.
 Gökhan DİKMEN, Okan USLU, et al. (2021). The application of qNMR for the determination of rosuvastatin in tablet form. Turkish Journal of Chemistry, (2021)45: 132-142.
 Chinese Pharmacopoeia Commission. (2015). Pharmacopoeia of the People’s Republic of China. Beijing China Medical Science Press, 2015: 52.
Determination of Absolute Content of Cyetpyrafen by qNMR Analysis
How to cite this paper: Jie Lang, Huibin Yang, Haibo Yu. (2022) Determination of Absolute Content of Cyetpyrafen by qNMR Analysis. International Journal of Food Science and Agriculture, 6(2), 149-153.